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BioTech_M.Sc Resume


Contact Candidate


Name:

Daisy Rani

Location:

India

Experience:

Willing to Relocate:

Willing to Travel:

Most Recent Job Title:

BioTech_M.Sc

Personal Website:

Objective:

I have completed my a final year (Last semi) M.Sc in Biotechnology in Bangalore. I have called for interview from IISc for Phd in biochemistry. Along with my study i have Completed training in Recombinant DNA technology,Genetic engineering, Plant tissue culture and Immunological techniques in Shreedhar Bhat's lab(bangalore). I have also done Diploma in Bio-Informatics from Noida. My topic "FUTURE OF NEUROSCIENCE" is published in journal "BIOINFORMATICS TRENDS". My essay "PATRIOTISM" got published in "GENERAL KNOWLEDGE REFRESHER" in October 2005.
My two projects are:

Project#1

Title : GENETIC MANIPULATION OF TOBACCO PLANT WITH ANNEXIN GENE THROUGH Argobacterium MEDIATED TRANSFORMATION.

Project#2

Title : IMMUNOTECHNOLOGY

My basic skills are



1. Blood group test, Agarose Gel Electrophoresis, ELISA, DNA isolation.

2. Testing sequence alignment using different techniques on internet.

3. Quantitative & Qualitative estimation of protein, carbohydrate, RNA & DNA.

4. Plant tissue culture.

5. Recombinant DNA technology.

6. Genetic engineering.

7. Immunotechnology





Resume Text:

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ve and an aspiring leader. Able to conduct hard work individually. In-depth understanding of emerging technologies and their commercial applications. Proficient use of MS-words tool kids, FASTA and BLAST, RASMOL, SQL, Perl and elementary knowledge of C-language. I am looking for well growth and challenging opportunities to exploit the inner talent in the best working environment for the emergence as a competitive professional so as to serve the very purpose of the organization.


Education Qualification


Msc in Biotechnology (persuing’08) 2006-2008 R.K institute of management
Bangalore
Ist sem 2007 69% R.K institute
II sem 2007 74% R.K institute
III sem 2008 75% R.K institute
Bsc in Zoology 2006 66% B.H college,Gauhati University, Assam
H.S.C Assam state Board 2002 61% Cotton college ,Guwahati, Assam
S.S.C Assam state Board 2000 78% St. Mary’s School, Assam


Skills:

 Blood group test, Agarose Gel Electrophoresis, ELISA, DNA isolation.
 Testing sequence alignment using different techniques on internet.
 Quantitative & Qualitative estimation of protein, carbohydrate, RNA & DNA. Related.
 Plant tissue culture.
 Handling PCR




Computer Skills:


SKILLS TOOLS
DATABASE SQL server, MS Access
Languages C, LOGO, BASIC, WORDSTAR, LOTUS123.
Operating System Win –98, Win –2000, Win –XP.
Software FASTA, BLAST, PERL, RASMOL.


Project Summary:
Project#1
Title : RECOMBINANT DNA TECHNOLOGY
Duration : 15 DAYS

Description:
 Basic handling:
a) Handling of equipments.
b) Basic calculations in molecular biology.
c) Media preparation and autoclaving.
 Growth and maintenance of bacteria carrying recombinant plasmid.
 Plasmid DNA isolation and treating it with RNase-A to get rid of RNA content.
 Restriction analysis.
 Gel elution of both vector and insert DNAs separately.
 Ligation reaction.
 Competent cells preparation and transformation.
 Competent cells efficiency testing using known quantity of DNA.
 Screening of transformants through α-complementation test.
 Polymerase chain reaction (PCR) machine handling and reaction.





Project#2
Title : GENETIC ENGINEERING
Duration : 45 days

Description: In addition to all the contents of project -1, this will have the following aspects.

 Screening of transformants by restriction analysis.
 Transferring the gene construct in binary vectors to Agrobacterium through tri parental mating (TPM).
 Transferring of gene to plant system through Agrobacterium mediated plant transformation.
a) Leaf disc preparation.
b) Co-cultivation.
c) Selection media transfer.
 Analysis of transgenic plants through PCR for confirming the presence of gene.
 Plant genomic DNA extraction.
 Polymerase chain reaction (PCR).


Project#3

Title : PLANT TISSUE CULTURE
Duration : 30 days

Description:

 Plant tissue culture media preparation and autoclaving.
 Cleaning and sterilization procedures.
 Isolation of embryos from the seeds and initiation.
 Sub-culturing.
 Synthetic Seed Preparation.
 Agrobacterium mediated plant transformation.
a) Leaf disc preparation.
b) Co-cultivation.
c) Selection media transfer.
 Leaf culture, nodal culture, embryo culture.

Project#4

Title : IMMUNOTECHNOLOGY
Duration : 15 days.

Description:

 Extraction of total proteins from different sources.
 SDS – PAGE for running protein samples from different sources including purified virus.
 Western blotting for immuno detection test.
 ELISA for testing samples for viral (ToMV) infection.

Achievements:
 My essay “PATRIOTISM” got published in “GENERAL KNOWLEDGE REFRESHER” in October 2005.
 Publication of topic “FUTURE OF NEUROSCIENCE” in journal “BIOINFORMATICS INDIA”.
 My 2 topics “BLACKHOLE” and “ADDICTION” got selected for wall magazine in consecutive years.
 Got 2nd prize in G.K competition n 3rd prize in quiz competition.
 Participated in “ASSAM UDYOD 2006” organized by Govt of ASSAM.
 Participated in the 3weeks “YOGA AND PHYSIOTHERAPY CAMP” in college.
 I was the part of COLLEGE UNION BODY in the year 2005-2006.
 I have been selected as the MISS FRESHER in the institute of my M.Sc last year.

Short-term Program:
Bioinformatics institute of India, Noida (U.P) (2003).

Diploma in bioinformatics Percentage: 72.00

Personal Details:
Name : Daisy Rani Nath
Address : C/o-Jitudhan Kumar, #905, 62nd Cross
50th Main, Kumarswami Layout, Bangalore-78.
Bangalore – 560093
Karnataka -India
Phone : +91-9986637919/ +91-9900594710
Email : Nathdaisy2005@gmail.com
Date of Birth : 20/04/1983


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