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Contact Candidate


Name:

sudeshna

Location:

India-West Bengal

Experience:

Willing to Relocate:

Willing to Travel:

Most Recent Job Title:

Wish to serve science

Personal Website:

Objective:

To work in an environment where innovation is the mantra and constant challenges will put my abilities to test and in that process enhance my knowledge and learning capabilities.
To be a part of a significant project where there is ample scope to showcase individual skills and thus gain knowledge which can be successfully applied in future to perform something radical and outright creative.

Resume Text:

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r /> Current Status: Senior Research Scholar
Year of submitting Thesis: going to submit thesis soon (2008)
Institute: Indian Institute of Chemical Biology

Key Skills:

Protein Chemistry: Protein purification and characterization, SDS-PAGE electrophoresis, Western blotting, N-terminal sequencing, Chemical modification of selective residues, Size exclusion chromatography, Ion exchange chromatography and Immune affinity chromatography.
Enzyme purification: Enzyme Purification includes Ultra filtration, Salt precipitation, Dialysis, Gel filtration chromatography, Ion exchange chromatography and High Performance Gel Permeation Liquid Chromatography (HPGPLC, WATERS, Ultropac TSK G2000 SW).
Enzymology and enzyme kinetics: General characterization of purified enzyme includes temperature & pH optima, temperature & pH stability, Amino acid Sequencing & Amino acid analysis, Km & Vmax, substrate specificity and effect of Trypsin (Proteolytic enzymes), Endo-H (N-Terminal Glycosylation determining enzyme), different salts, and chaotropic agents on purified enzyme.
Immunology: Simple-Immunodiffusion, Immunodiffusion ring test, Immunoelectrophoresis, Rocket electrophoresis, Indirect Immuno labeling of cultured cells, Immuno precipitation study, & Western blotting.

Spectroscopy: UV-Vis, Fluorescence & C.D (Circular Dichroism) analysis.

Bioinformatics Skills: Database searching and homology analysis with BLAST, SWISS-PROT etc. servers.

Microbiology skills: Isolation of aerobic bacteria from soil, Gram charecterisitics,Isolation & enrichment of heat resistant bacteria,endospore staining of heat resistant bacteria, determination of antibiotic sensitivity of soil bacteria, simple staining of yeast, determination of minimal inhibitory conc. of detol against bacteria, Growth curve of bacteria, Microbial estimation of water(collected from various field). Aseptic culture preparation of microorganisms, basically fungal culture preparation and Leishmanial culture preparation.

Biophysics: Determination of concentration of proteins by using spectrophotometer, Absorption spectroscopic study of proteins, Fluorescence spectroscopic study of proteins at different pH, CD spectroscopic analysis of proteins at different pH, Mass spectroscopic analysis of purified protein for determination of protein mass and HPGPLC.

Cell biology: Determination of role of Glycosylation inhibitors [Tunicamycin, 2DG & Gluconolactone] on fungal enzyme activity and production and Chaperon-like activity of the fungal enzyme, Isolation of mitochondria, Mitochondrial potentiality measurement from Leishmania donovenii, TdT labeling of mice grem cells.

Computer Knowledge :

Basic knowledge in
 Microsoft Office Applications,
 Adobe Photoshop,
 Microsoft excel &
 Power point presentation
Extra curricular activities:

Abstracts and presentations:

 2002: Successfully participated in the Patent Awareness Workshop organized by THE PATENT OFFICE, KOLKATA in collaboration with the National University of Juridical Sciences, kolkata, held in the Paribesh Bhavan at Salt Lake City on 27th Feb’2002.


 2003: Participated in poster presentation session on 6th INTERNATIONAL SYMPOSIUM ON BIOCHEMICAL ROLES OF EUKARYOTIC CELL SURFACE MACOMOLUCULES held on 16-18th January 2003 at Indian Institute of Chemical Biology, Kolkata.

 2006: Participated in poster presentation session on TEACHING, RESEARCH & EXPLORATION IN BIOCHEMISTRY: 50 Years of Journey held on 6-7th January, 2006 at Ballygunge Science College Campus, University of Calcutta.

Publications:

 “Cellobiase from Termitomyces clypeatus: activity and secretion in presence of glycosylation inhibitors” Biotechnology Letters, 28, 1773-1778, 2006: S. Mukherjee, Sudeshna Chowdhury, S. Ghorai, S. Pal, Suman Khowala.

 Manuscript of paper entitled “Purification and Characterization of low molecular weight sucrase from Filamentous fungus Termitomyces clypeatus” has been communicated to BBA.

Patents:

 Indian patent entitled “A low molecular weight polypeptide with hydrolyzing activity on beta-D-fructofuransyl-alpha-D-glucopyranoside” has been submitted to CSIR on May, 2008.Filling number will be received soon.

 International patent entitled “Molecular chaperon-like properties of a low molecular weight polypeptide with beta-fructofuranosidase activity” has been submitted to IICB patent Department on 20th May,2008, will be submitted to International Patent Department soon.


Thesis:

Field: Biotechnology & Proteomics

Title: “Co-aggregation of sucrase with cellobiase in the regulated secretory pathway of filamentous fungus Termitomyces clypeatus”

Supervisor: Dr. Suman Khowala.
Drug Development & Biotechnology.
Indian Institute of Chemical Biology, Kolkata, India

Duration: June, 2002-till date.

Project:

Field: Biochemistry & Cell biology

Title: “The studies on the biochemistry of Leishmania donovenii whole cells and mitochondria”

Supervisor: Dr. Chandrima Shaha.
Sperm Biotechnology Department.
National Institute of Immunology, Delhi, India.

Duration: May, 2001-July, 2001.

Educational Qualification:
 2002-Present: Doctoral Scholar. Indian Institute of Chemical Biology, Department of Drug Development and Biotechnology, going to submit thesis soon. Thesis title “Co-aggregation of sucrase with cellobiase in the regulated secretory pathway of filamentous fungus Termitomyces clypeatus”.

 2000-2002: Master of Science [70.7%] Subjects- Biotechnology and genetic engineering; University of Calcutta, Department of Biotechnology.

 1997-2000: Bachelor of Science [70.5%] Subjects- Microbiology, Chemistry, Zoology, English; Dinabandhu Andrews College, University of Calcutta.

 1995-1997: Higher Secondary Examination- [65.5%]: Subjects- Chemistry, Mathematics, Physics, Biology, English, and Bengali. Jodhpur park girl’s school, West Bengal Board of Higher Secondary Education.

 1985-1995: Secondary Examination- [80.56 %]: Subjects- Physical science, Life Science, Mathematics, English, Bengali, History, Geography and Work Education., Jodhpur park girl’s school, West Bengal Board of Secondary education.

Professional Summary:

 Good conceptual knowledge about Microsoft Word, Microsoft Excel, Microsoft power point, Networking Fundamentals, C language.

 Has the motivation to take independent responsibility as well as ability to contribute and be a productive team member.

Achievements:

 2003-2004: Successfully passed IICB PhD course work program.

 2002: Awarded DAE sponsored scholarship to conduct Ph.D. thesis research on “Co-aggregation of sucrase with cellobiase in regulated secretory pathway of filamentous fungus Termitomyces clypeatus”, under the supervision of Dr. Suman Khowala, Department of Drug Development and Biotechnology, Indian Institute of Chemical Biology, Kolkata - 32.

 2002: Qualified GATE-2002 (General aptitude test in engineering) organized by department of education, Ministry of human resource development, Govt. of India.

 2000: Recipient of National Scholarship under the National Scholarship Scheme (1999-2000) on the result of B.Sc (Microbiology), Ministry of Human Resource Development, Government of India

 1995: Recipient of National Scholarship under the National Scholarship Scheme on the result of Secondary Examination, West Bengal Board Secondary Education.


Membership of Learned Society:

Life membership of Society of Biological Chemists (SBC).

Strength and abilities:


 I am a quick learner and can pick up things very fast.
 I can adapt myself to the changing circumstances as well.
 I like to work in a team.











Personal Details:

 Date of Birth: 03/06/1978.

 Father’s Name: Mr. Jaladhar Chowdhury.

 Marital Status: Married.

 Husband’s Name: Mr. Arunasis Adhikari.

 Permanent Address : 4, South Santoshpur
Ghoshpara, Jadavpur
Kolkata- 700032.

 Language Known: English, Bengali, Hindi.

 Nationality: Indian.

 Hobbies: Games, Music, Drama.

Declaration:

I hereby declare that the above mentioned information is true to the best of my knowledge and belief.


(SUDESHNA CHOWDHURY)


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