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Dhanya
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Freshser
To work in a challenging and competitive environment to improve my knowledge and where my capabilities can be put to use for the development of the organization
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entage (%)
M. Tech
Biotechnology VIT UNIVERSITY, Vellore,TN 2006-2008 9.2 CGPA
(Till 3rd semester)
B.Tech
Biotechnology Met’s School Of Engineering, Mala
(Affiliated to Calicut University, Kerala) 2002-2006 78
XII Kendriya Vidyalaya,Cochin 2001 80
X Kendriya Vidyalaya,Cochin 1999 80
Projects Undertaken
• Project on Expression of HPV L1 capsid protein gene in model system-Nicotiana tabacum at Rajiv Gandhi Center for Biotechnology, Trivandrum
Responsibilities: Cloning of HPV L1 gene into pGEM-T vector, Cloning of HPV L1 gene into pRT 101,Cloning of Plant expression cassette into pCAMBIA 3300,Triparental mating, Agrobacterium mediated gene transfer into tobacco leaf discs, Confirmation of the presence of
HPV L1 gene in tobacco plants by plant DNA isolation and PCR amplification
• Project on Antimicrobial properties of medicinal plants at VIT University,Vellore
Responsibilities: Sample preparation using Tamarindus indica, Amorphophallus paeoniifolius,Cajanus cajan and Dolichos biflorus , Solvent extraction from sample using soxhlet apparatus, Concentration of the extract using rotary evaporator, Descication of the concentrated sample ,Testing for antimicrobial activity
• Project on Recovery of bioactive components on Lactospore® fermentation supernatant at Sami Labs Ltd, Bangalore
Responsibilities: Collection of supernatant, Clarification of supernatant using microfiltration followed by ultrafiltration, Organic solvent mediated precipitation of proteins, recovery of
precipitated protein by centrifugation, Drying of precipitated proteins using rotavapour,Estimation of total protein content, total amount of reducing sugar, amylase activity, protease activity and lactase activity for supernatant, microfiltration permeate, ultarfiltration permeate and retentate and
precipitated protein.
Techniques Known
• Biochemical assays
• Nucleic acid, reducing sugar, amino acid and Protein estimation
• Agarose gel electrophoresis
• Elisa
• PCR
• Genomic and plasmid DNA isolation
• Immunological Techniques like Immunodiffusion, Immunoelectrophoresis,Counter
Immunoelectrophoresis, Immunoprecipitation, Immunopurification,Latex agglutination
• Competent Cell preparation
• DNA gel elution and purification
• Bacterial Transformation
Area of interest
• Genetic Engineering
• Bioprocess Engineering
• Downstream Processing
• Chromatographic Separation
• Bioremediation
Languages known:
Read and write : English, Hindi and Malayalam
Speak : English, Hindi and Malayalam
Personal Details
Date of Birth : 28 Nov 1983
Gender : Female
Nationality : Indian
Martial Status : Married
Husband’s Name : Mr. Ajeesh Mathew
Permanent Address : Vadakketh adakkethu Aarakulam P.O, Moolamattam, Idduki
Contact Number : +919900833779
References
1. Dr. Lazer Mathew, Dean, School of Biotechnology, Chemical and Biomedical Engineering, VIT University,Vellore
2. Dr. Soniya .E.V, Scientist, Plant Molecular Biology lab, Rajiv Gandhi Canter for Biotechnology, Trivandrum
DECLARATION
I here by declare that the above given information’s are true to my knowledge and consent
Place: Bangalore
Date: 30.06.2008
DHANYA CHACKO
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