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Pratibha
India-Rajasthan
1-3 yrs
biotech or microbiology
To work in well disciplined manner with full dedication and to deliver the best results by properly utilizing my skills, knowledge and the resources available, and to explore the hidden aspects of life sciences by implementing my knowledge and skills.
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l Education (Rajasthan Board)
Percentage marks obtained – 69%
Secondary School Education (Rajasthan Board)
Percentage marks obtained – 75%
Experimental skills:
Biochemistry: Quantitative estimation of DNA- Dipheny lamine method, RNA- Orcinol method, Protein- Lowry’s, BCA, Bradford’s and TCA method, Carbohydrates- reducing and non reducing sugar, Fats- Cholesterol, Isoelectric focusing of proteins.
Genetic Engineering: Isolation of plasmid DNA from E.coli, electrophoresis (agarose gel) & its restriction digestion, extraction of DNA & RNA from plant cell.
Enzymology: preparation of enzyme extract, determination of kinetic properties (Km & Vmax), enzyme purification by salt precipitation, immobilization via entrapment, determination of activity.
Immunology: ELISA-determination of antibody titer, double immunodiffusion, Immunoelectrophoresis, Western Blotting.
Instrumentation: Centrifuge, pH meter, Spectrophotometer, separation of Proteins by SDS-PAGE, Tris-Tricine SDS-PAGE
Microbiology: Measurement of bacterial & fungal growth, isolation & enumeration of microorganisms from air & soil by agar plating, antibiotic sensitivity test
Cell & tissue culture: Embryo & explants culture.
Bioprocess technology: Estimation of growth & product yield in a bioconversion process, enzyme biosynthesis & measurement of it’s activity.
Chromatography : Paper, TLC, HPLC
Industrial Training
Has undergone one month training at ZYDUS CADILA Health care Ltd. Moraiya, Ahemdabad in Quality Control Department.
Project dissertation (4 months) from ZYDUS RESEARCH CENTER, Ahmadabad in protein purification lab on “Physicochemical properties of a recombinant therapeutic protein produced in the form of inclusion bodies in E.coli.”
Computer Proficiency
Operating System: Windows
MS Office – Excel, PowerPoint, Word, Access, Outlook, Internet Explorer
Other Softwares: Auto-CAD, DBMS
Achievements
3rd rank holder of College in B.Sc.
Adjudged with Rajaypal Award in Scout Guiding
State level player of Table Tannis
Has been Volunteer of N.S.S for three years.
Student member of Indian Science Congress Association.
Area of interest
Biochemistry
Immunology
Recombinant DNA technology
Environment Biotechnologys
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