research and production in biotechnology Resumes in India-Gujarat: Khushi

research and production in biotechnology Resume

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Name:

Khushi

Location:

India-Gujarat

Experience:

Willing to Relocate:

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Most Recent Job Title:

research and production in biotechnology

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Objective:

Have a knowledge of biotechnology and microbiology work.

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English 53.90
M.Sc. (Biotechnology) Saurashtra University 2008
(Degree awaited) English 57.69

Dissertation M.Sc. dissertation entitled “Study of Polymorphism in beta-defensin gene using PCR-RFLP in cattle breed” at ANAND AGRICULTURE UNIVERSITY, ANAND

Participation in National Symposium/Seminar

• Oral Presentation on “ Chloroplast Genetic Engineering – For Green Future” at National Level Science Symposium 2008 organized by Christ College, Rajkot, Gujarat

• Oral Presentation on “Nanotechnology” in UGC Sponsored National Level Symposium Atmiya SciTech Utsav 2006 held at Virani Science College, Rajkot, Gujarat
• Article Writing on “Gravitational Biology” in UGC Sponsored National Level Symposium Atmiya Scitech Utsav 2006 held at Virani Science College, Rajkot, Gujarat

Experimental skills
• In vitro callus induction & Regeneration of Plants
• Isolation of DNA from Plant source, Mammalian blood & Bacterial genomic and Plasmid DNA
• Agarose & Polyacrylamide Gel Electrophoresis
• DNA elution from Gel
• Polymerase chain reaction (PCR)
• RAPD & ISSR Analysis
• Restriction digestion of plasmid with restriction endonuclease and its analysis on agarose gel
• Ligation of DNA by T4 DNA ligase
• Bacterial artificial transformation
• Isolation and identification by selective and differential media.
• Primary screening
• Isolation and characterization of Halophiles, Heavy metal accumulating & Dye degrading oraganisms
• Isolation of DNA from halophiles
• Biochemical tests for proteins and sugar
• Estimation of Proteins, Sugar & Alcohol
• Enzyme Kinetics
• Protein Precipitation, Dialysis & Protein Purification
• Chromatographic techniques such as Ion Exchange Chromatography, Affinity Chromatography, Paper Chromatography, Thin Layer Chromatography, Gel Filtration Chromatography & Solvent -Solvent Extraction
• Immobilization
• Alcohol fermentation
• Total count & Differential count of RBCs and WBCs
• Blood Grouping
• Immunological techniques such as ELISA, SRID, ODD, Widal test & Latex Agglutination
• BOD, COD & DO Analysis
• TS, TDS & TSS Analysis
• Phosphorus-phosphate & Nitrate-N, Nitrite-N and Ammonium-N Estimation
Bioinformatics skills

• Molecular data analysis for studying genetic diversity using NTSyS software
• Primer designing using Primer 3 & Oligos software
• Information retrieval using Bioinformatics databases
• tools such BLAST, FASTA, KEGG, Align, Clustal W, Glimmer, ORF Finder, GenScan, VAST, Modeller, SOPMA etc
Work Experience:-
Worked as a trainee at ANAND AGRICULTURE UNIVERSITY under the project ‘Study of Polymorphism in beta-defensin gene using PCR-RFLP in cattle breed’ for 2 months.
Declaration
I hereby declare that all the above information is true to my knowledge and belief.

(Khushi A.Ruparel)
Academic References
1 Dr. Prashant K. Firke,
Lecturer, Department of Biotechnology, Sh. M. & N. Virani Science College, Rajkot-360005, Gujarat.
Cell Phone: +91-9974344686
Email: pkfirke@rediffmail.com

2 Ms. Shivani Patel,
Head, Department of Biotechnology, Sh. M. & N. Virani Science College, Rajkot-360005, Gujarat.
Cell Phone: +91-9825394037
Email: vsc_bt@rediffmail.com

3 Dr. C.G. Joshi
Head, Department of Biotechnology , Veterinary Science College,
Anand Agriculture University,
Anand, Gujarat
Phone: 0269 - 261201
Email: cgjoshi@rediffmail.com

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