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ritu
India-Andra Pradesh-Hyderabad
research
to use my molecular and genetic shills for crop improvement
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.act.in) Title: “Studies on micropropagation of strawberry cultivars Chandler and Fern through vegetative buds.”
• B.Sc. (Medical), 1993-1995, Botany, Chemistry and Zoology as main Subjects, first Class (74%) Himachal Pradesh University-Shimla (HP) India
WORK EXPERIENCE: ( 8 years)
1. Research Associate
• National Bureau Of Plant Genetic Resources,
New Delhi
Since December, 2000 – June, 2003
Worked in DBT funded project Entitled “Standardization of different cryopresevation techniques for long term preservation of medicinal Yams (Discorea sp).”
• Central Potato Research Institute,
Shimla
Since December, 2000 – June, 2003
Worked in DBT funded project entitled “Chemical induction of apospory in potato”
May 1998 to July 2000
2. Senior Research Fellow
• Punjab Agricultural University,
Ludhiana
Worked in DBT funded project entitled “ Identification, functional characterization and utilization of Bacterial leaf blight resistance genes in rice”
March 2005 to March 2007
• Dr YS Parmar University of Horticulture. & Forestry.
Nauni, Solan (HP)
August 1997 to Dec 1997.
Worked as Senior Research fellow in DBT funded Project entitled “Micropropagation of Robinia sp.”
TECHNICAL SKILLS:
For the last eight years I have been working in molecular biology and tissue culture research projects. I have gathered skills in the following techniques:
Molecular Biology:
• Isolation of plasmid and genomic DNA from plants, Fungus
• Electrophoresis - Agarose gels, Polyacrylamide gels
• PCR (Microsatellite Markers and RAPDs)
• Cloning of DNA
• Designing Of primers
• Preparation of heat shock and electro competent cells
• Transformation in E.coli using Heat shock and Electroporation methods
• Sequencing of DNA (LICOR)
• Spectrophotometry
• Knowledge of operation of instruments and protocols of basic chemical solutions used in molecular lab.
Tissue Culture :
• Experience in in vitro tissue culture in Rice, Gladiolus, Allium, Cicer Sweet potato, Alocacia and Dioscores sp. Kufri jyoti, K.giriraj, K. chandermukhi, K.bahar, K.pukhraj, Chipsona I and Chipsona II (all potato varieties), Robinia and Morus alba sp.
• Establishment, Multiplication, rooting, hardening etc. regeneration of plantlets,
callus culture, somatic embryogenesis, embryo culture, embryo rescue, meristem culture, fungal culture, bacterial culture.
Invitro culture and Cryopreservation:
(both dehydration and encapsulation techniques)
Preparation of synthetic seeds using calcium alginate
Computer Skills:
• MS office, Adobe
• NTSYS, Mapmaker, Graphical Genotyping
Awards
• Best student award in school
• HPU scholarship for consecutive three years in graduation
• University Stipend in M.Sc.
• Part time fellowship by DBT in Ph.D program
Training Attended
Training Course in “Genomics and Genome Sequencing” held from 16-26 Nov, 2004 in the Department of Plant Breeding, Genetics and Biotechnology, PAU, Ludhiana. Punjab, India
LIST OF PUBLICATIONS
Papers published
R Kaur, Ritu Mahajan, S V Bhardwaj and D R Sharma (1998) Elimination of Strawberry Mottle Virus through shoot meristem culture In: Proceedings of National Symposium on Biotechnology and Environment (25-26 March) at ITTT, by Punjab State Council for Science and Technology, Chandigarh, 116-19.
R. Kaur, M Sood, S Chander, R Mahajan, V Kumar and D R Sharma (1999) Invitro propagation of Valeriana jatamancii- a medicinal plant. Plant Cell Tissue and Organ culture 59: 227-29.
Ritu Mahajan, B P Singh, Ela Atheaya, P H Singh, Brajesh Singh and G S Shekhawat (2000) Leaf attributes as modifiers of resistance to Late Blight in Potato In: Potato, Global and development, Vol I . Indian Potato association, Shimla pp 608-10.
R Kaur, Ritu Mahajan, S V Bhardwaj and D R Sharma (2000) Meristem tip culture to eliminate strawberry mottle virus from two cultivars of strawberry. Phytomorphology 50: 192-94.
Ritu Mahajan and R Kaur (2000) Effect of size of shoot meristem on its survival and virus elimination in two strawberry cultivars Chandler and Fern. Res on Crops 1: 379-381.
Ritu Mahajan, R Kaur, S V Bhardwaj and D R Sharma (2000) Micropropagation of strawberry cultivars chandler and Fern. Crop Improvement 28: 19-25.
Ritu Mahajan (2007) Effect of different sterilants on surface sterilization of explants in two strawberry cultivars i.e. Fern and Chandler J of Plant Science Research 22: 203-06.
R.Kaur, Sadiq Majeed, V Kumar, Ritu Mahajan, Bhawna Saxena and D R Sharma (2007) Invitro propogation and conservation of Gentiana kurroo-a temperate medicinal herb. J of Plant Science Research 23: 69-72.
Kuljit K Cheema, Navjit K Grewal, Yogesh Vikal, Aparna Das, Rajiv Sharma, Jagjeet S Lore, Dharminder Bhatia, Ritu Mahajan and Kuldeep Singh (2007). A new bacterial blight resistance gene Xa30(t) from Oryza nivara (Sharma et Shastry) mapped to 176kb region and transferred to O. sativa L. Mol Breeding (under revision).
Conference Presentations (Abstracts):
Ritu Mahajan (1998) Invitro propagation of Strawberry cultivars Chandler and Fern through vegetative buds In: 50 years of Himachal Horticulture (16-17 Nov) Shimla.
Ritu Mahajan, B P Singh, Ela Atheaya, P H Singh, Brajesh Singh and G S Shekhawat (2000) Leaf attributes as modifiers of resistance to Late Blight in Potato In: Proceedings on Global Conference on Potato (6-10 Dec), Delhi, India.
Ela Atheaya, Ritu Mahajan, B P Singh, P H Singh, Brajesh Singh and G S Shekhawat (2000) Population Dynamics of A1 and A2 mating types in Phytophthora infestans In: Proceedings on Global Conference on Potato (6-10 Dec), Delhi, India.
Kuljit K Cheema, Ritu Mahajan, Navjit Grewal, Aparna Das, Yogesh Vikal, Jagjeet Singh, R K Goel and Kuldeep Singh (2007) Molecular mapping and transfer of three new Bacterial Blight resistance genes from Oryza nivara, O. glaberrima and O. barthii to O. sativa In: “National seminar on Molecular Approaches for Crop Improvement” held at Nagendra Dev University of Agriculture and Technology, Nagewndra Nagar (Kumarganj), Faizabad, India 7-8 Feb 2007.
Kuldeep Singh, Y Vikal, K Cheema, GS Mangat, A Das, S Joshi, D Bhatia, R Mahajan, R Kaur, N Grewal (2007) Integrating Molecular Markers and Wide Hybridization for improving productivity and its sustainability in Indica and Basmati rice In: 2nd International Conference on Plant Molecular Breeding, Sanya, China, 23-27 Mar 2007.
PARTICULARS OF RESEARCH WORK DONE
• Mapping of Bacterial Blight (BB) resistance gene from two accessions of wild rice O. nivara in the background of cultivated rice varieties (O.sativa).
Two BB resistance genes from O. nivara Acc. 81825 and Acc. 100428 have been transferred to cultivated variety PR114 (O. sativa). Morphological studies on BC2F1 population suggested that Acc. 81825 provides single dominant BB resistance gene where as Acc. 100428 provide recessive gene for resistance to bacterial blight. The gene from Acc.81825 has already been mapped on Chromosome 4 and is mapped to 156 kb region and also is transferred to O. sativa whereas mapping of BB resistance gene from Acc. 100428 is in progress.
• Mapping of Bacterial Blight (BB) resistance gene from two accessions O. glaberrima (Acc. 102600b) and O. barthii (Acc. 100119) in the background of cultivated rice varieties (O.sativa).
Two new BB resistance genes from the two accessions O. glaberrima (Acc. 102600b) and O. barthii (Acc. 100119), have been transferred to cultivated variety Pusa44(O. sativa). Inheritance studies on BC1F1 and BC2F1 populations indicated that resistance to bacterial blight is governed by a single recessive gene. The BB resistant gene from O. glaberrima is fine mapped on chromosome 5 at a distance of 1.1cM on the short arm and in O. barthii on chromosome 6 at the distance of 9.3cM on the terminal end of short arm. Its fine mapping is in progress whereas for O. glaberrima gene cloning is in progress.
• Bacterial culture and DNA fingerprinting
Culturing of Xanthomonas strains both in solid and liquid media. Their diversity analysis using RAPD primers.
Embryo rescue from different wild species of rice viz O. australensis, O. punctata, O. officinalis all belonging to tertiary gene pool
• Invitro conservation and cryopreservation
Invitro conservation of Gladiolus, Allium, Cicer Sweet potato and Dioscores sp. Using different media and conditions and Cryopreservation of different Dioscorea sp. using dehydration and encapsulation techniques
• Fungal culture, DNA fingerprinting and bacterial culture
Isolation of Phytophthora infestans from tubers and leaves and their purification and their characterization using RAPD primers so as to study their diversity at different places.
Studing how different leaf attributes like no. of stomata, epicuticularwax, leaf hair helps in providing resistance to plant against disease.
• Micropropagation
Successful micropropagation of Kufri jyoti, K.giriraj, K. chandermukhi, K.bahar, K.pukhraj, Chipsona I and Chipsona II (all potato varieties), Robinia and Morus alba sp.
Studing apospory in potato using different chemicals
BRIEF ACCOUNT OF Ph.D WORK:
Bacterial Blight (BB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the major diseases of rice. One accessions each from O. glaberrima (IRGC 102600b) and O. barthii (IRGC 100119) carrying BB resistant gene was used for studying inheritance, mapping and its transfer to O. sativa cv Pusa44. Pusa44 is known for its high productivity, but at same time it is susceptible to bacterial blight.
Till now there is no report where any BB resistant gene has been designated from these two African species, O. glaberrima and O. barthii.
The F1s of these accessions indicated that the BB resistance genes in O. glaberrima and O. barthii are recessive in nature which was reconfirmed in BC1F1 and BC2F1 generation as well.
• Inheritance studies showed that resistance in these two accessions was governed by a single recessive gene.
• The two BCIF2 populations, one each with O. glaberrima and O. barthii were used for mapping of BB resistance genes. Polymorphism level of 55.2% and 51.3% was observed in cross between O. glaberrima and Pusa44 and O. barthii and Pusa44 respectively.
• BSA indicated presence of O. glaberrima resistance gene on chromosome 5S and that of O. barthii on chromosome 6S of rice.
• Analysis of population with SSR markers in the region defined by BSA was carried out for both the populations. The BB resistance gene in O. glaberrima mapped on chromosome 5S flanked by markers RM548 and RM593 at a distance of 1.7 cM and 1.1 cM respectively.
• The BB resistance gene in O. barthii on terminal end of chromosome 6S was linked to RM 588 at a distance of 9.3 cM.
• This is the first study of BB gene transfer from O. glaberrima and O. barthii to cultivated species and its mapping on the chromosome and both the genes seem to be novel.
REFEREES:
Dr. Kuldeep Singh
Molecular Geneticist
Department of Genetics
Punjab Agricultural University
Ludhiana-141004
Telephone: +91-161-2401444(Office) Fax: +91-161-2401444; +91-161-2400945
Email: kuldeep35@yahoo.com
Dr. Rajinder Kaur
Professor
Department of Biotechnology,
Dr YS Parmar University of horticulture and Forestry,
Nauni, Solan (HP
Telephone: +91-0-9418151645 (M)
Email: rkaur_uhf@rediff.com
Dr. K. Kumar
Associate Professor
Deptt. Of Fruit Breeding.
Dr YS Parmar University of horticulture and Forestry,
Nauni, Solan (HP
Telephone: +91-0-9418020518 (M)
Email: dr krishankumar@gmail.com
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