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pavitra
India
Lecturer
To work in healthy and challenging environment so that I can utilize my knowledge in a better way and can contribute in the development of organization.
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matics Department of Mata Gujri College (P.G), Fatehgarh Sahib affiliated by Punjabi University, Patiala.
Qualification:
• I have Qualified NET exam in previous year.
Qualification Year School/College University/Board/Society Marks
M.Sc. (Bioinformatics) 2007 Basic Sciences & Humanities, Hisar CCS Haryana Agricultural University, Hisar 76.10 %
B.Sc.
(Life Sciences) 2005 Pt. J.L.N College, Faridabad Maharshi Dayanand University, Rohtak 66.00 %
XII 2002 Kurukshetra Sr. Sec. School, Faridabad H.B.S.E 74.20 %
X 2000 Nivedita Shiksha Sadan, Faridabad H.B.S.E 68.72 %
Technical Skills:
Operating Systems DOS, Windows ,Unix
DBMS MS-ACCESS, MYSQL
Languages C, Visual Basic, Perl, HTML, XML, PHP
Scientific Softwares Rasmol, Chimera, Swiss PDB Viewer, Hex, Auto dock, Chemdraw, ISIS, ChemOffice, EMBOSS, ChemSketch
Various Bioinformatics Tools Structure Prediction tools, Homology Modelling, Phylip package, Mega3.1, Bioedit, Tree view, Swiss Model Server, GENSCAN, Cn3d, Fgenesh, Grail, Openbabel, SRS, Entrez, BLAST, FASTA, ClustalW, ClustalX, MSA, TCOFFEE.
Other Computer Related Skills: Microsoft-word, PowerPoint, Excel and Adobe Photoshop.
Project Done: My project work during M Sc. Entitled as -
“Rice Genome Search for BAD-2 Homologus Genes”
Introduction: - Rice aroma is shown to be linked to BAD-2 locus with a recessive gene at chromosome 8 having eight bp deletions. This recessive gene largely controlling the level of 2-acetyl-1-pyrroline and several genes may have more than one copy of the genome. So it was very interesting to see weather the rice genome also possess the intact copy of the BAD-2 somewhere else in the whole of its genome. Keeping this consideration in view, whole of this project was proposed with the following objectives:
• To search the whole rice genome for BAD-2 homologous of genes.
• To search the genome database for BAD-2 homologous of genes in other crops.
Methodology: - Nucleotide sequence of BAD-2 gene which is present on chromosome no. 8 of rice genome was retrieved from NCBI (www.ncbi.nlm.nih.gov/). Using this nucleotide and locus information about BAD-2 the whole sequence for BAD-2 was retrieved.BAD-2 sequence was taken as query sequence and by using blastn program alignment of this query sequence with the rice genome was done. So that most of the homologous DNA sequences against Oryza sativa database were returned. Multiple sequence alignment was done with nucleotide sequences of 30 accessions for the BAD-2 gene from various crops retrieved from the ENTREZ Nucleotide Database of NCBI. Then results from complete alignment were treated for further Phylogenetic analysis using ClustalX, PHYLIP and Tree View. BAD-2 sequence is then subjected to CDD to view all conserved domains associated with it.
Results and Discussion: - BAD-2 gene is expected to have 15 exons. Five hits at chromosome 4 also belong to the gene for BAD-1 which is a homologous gene to BAD-2. As a result BAD-1 locus at chromosome 4 was showing 77.08% homology to BAD-2. The sequence for recessive BAD-2(fgr gene) was also retrieved and subjected to pair wise alignment by blast using BAD-2(non fragrant) as query sequence. This shows 96.37% homology. Phylogenetic analysis of 30 accessions from various crops using BAD-2 as keyword was also done. This observation suggests that this type of fragrance may also be found or produced by other plant species. The conserved domain of BAD-2 sequences was determined using CDD. The domain matches to the other known aldehyde dehydrogenase protein domains.
Software Used: FGENESH, GENSCAN, Phylip, MEGA 3.1, Clustal package, Bioedit, Tree view.
Seminars Delivered:
Pharmacogenomics, Genomics and Proteomics, Bioinformatics in Disease Diagnosis and Homology Modeling of Proteins.
Personal Information:-
Name Pavitra Kumari
Father’s Name Dharampal Singh
Date of Birth May 23, 1985
Gender Female
Marital status Single
Contact No. 9999427851,9888557520
Languages Known English, Hindi
E-Mail ID pavitra.bioinfo@gmail.com
References:-
1. Dr. Sudhir Kumar
Associate professor, Coordinator and HOD Bioinformatics Section, College of Basic Sciences and Humanities, CCSHAU, Hisar-125004. Contact No-09354320276
Email: - sudhir@hau.ernet.in
2. Dr. Sunita Jain
Associate professor Deptt of biochemistry, College of Basic sciences and Humanities, CCSHAU, Hisar- 125004. Contact No-09466062460
Email: - Sunita_jain@hau.ernet.in
3. Mr. O.P. Sheoran
Advisor and professor, Computer section, College of basic sciences and humanities, CCSHAU, hisar-125004
Email: - opsheoran@hau.ernet.in
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