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divya
Australia
GENETIST
Seeking a scientific and research based position in the field of molecular genetics, Biotechnology and in a professional organization, working under strict deadlines and schedules.
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king on project “Role of Leptin in Polycystic Ovary Syndrome (PCOS)” and “Chromosome Abnormalities: Genetic Images of Life” in Centre for Cellular and Molecular Biology (CCMB), Hyderabad.
ð Designated as Guest worker with CCMB and working on project called “Establishment of Reptilian cell lines and subsequently preparing snake specific chromosome painting probes”.
ð Possesses excellent interpersonal skills that have been put to good use while interfacing with researchers /scientists for ascertaining requirements and delivering need based results.
Organisational Experience
Work experience from July-November 2007 with Centre for Cellular and Molecular Biology (CCMB), Hyderabad as Guest worker.
Broad Performance Areas
“Establishment of Reptilian cell lines and subsequently preparing snake specific chromosome painting probes”
Supervisor
Mrs. Lakshmi Rao (Dr. Lalji singh’s group), CCMB, Hyderabad, India
Key Responsibilities
Establishment of primary cultures from water snakes and maintenance of the same. Immunostaining to check types of cells present in the culture and, limiting dilution to get specific cell type cell lines. Chromosome preparation from these cells lines and subsequently using FACS to sort these chromosomes for snake specific painting probes.
Dec 2006 to May 2007 as “Intern” (MSc, Dissertation), with Mrs. Lakshmi Rao (Dr. Lalji singh’s group), CCMB, Hyderabad, India.
Project
1. Chromosome Abnormalities: Genetic Images of Life
Work Summary: Chromosomal rearrangements are associated with infertility and mental retardation. Our study is dealing with different infertility and mental retardation cases. Each case is showed an interesting association with a particular phenotype. Chromosomal rearrangements are identified by the conventional banding technique GTG banding and for confirmation we used fluorescent insitu hybridization, NOR staining, Acro p arm staining.
2. Role of Leptin in Polycystic Ovary Syndrome (PCOS)
Work Summary: Leptin having an imporatnt role in the regulation of body fat and food intake which is associated with obesity. PCOS is associated with increased androgen, insulin level and infertility. Some PCOS patients are showing increased level of leptin and obesity. The present study was aimed at investigating the genetic relation between the leptin receptor gene (LEPR) and PCOS using polymerase chain reaction followed by sequencing. A case-control-based study approach was used, which included 102 controls, 50 lean PCOS and 60 obese PCOS from the Indian women population
Summer trainings
Insilco drug designing using QSAR with Indian institute of chemical biology (IICB) Kolkata, under the guidance of Dr. Nanda Ghoshal.
Summary of the work: Designing of small molecules on the Hyper-chem software and ACD chemskech (PC based software) and Cerius2 module (SGI machine, IRIX based software) this included modeling and energy minimization to obtain the least energy conformation. Also included modeling of few compounds for carrying out QSAR studies of some anti-convulsant compounds (some part of the work being carried out in the lab) using QSAR related modules of Cerius2 software and minimization the same using Smart Minimization option.
Other Professional trainings
1. On Job Training in Genetic Engineering at Institute of Bioscience and Molecular Biology (IBMB), Bangalore under the guidance of Dr.T.T.S.Ramachandra Murthy and Denny John.
2. On Job Training in Isolation of Industrially Important Enzymes from Microorganism in the department of Industrial Microbiology at Jain Institute of Vocational and Advanced Studies (JIVAS), Bangalore under the guidance of H.G.Mohan Kuma
Technical Experience
· Nucleic Acid Isolation (DNA and RNA)
· Electrophoresis
· PCR and DNA sequencing
· Genescan
· Karyotyping of human chromosome
· Fluorescent insitu hybridization, NOR staining
· Cell line culture
· FACS
· Immunostaining
Academic Credentials
Master of Science in Biomedical Genetics, CGPA 8.29 / 10
Vellore Institute of Science; VIT University ,Vellore, T.N.
Personal Details
Name : Divya Rose T.G
Date of Birth : 24.05. 1985
Sex : Female
Nationality : Indian
Marital Status : Single
Religion : Christian
Languages Known : English, Malayalam
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