Research and Development-Microbilogist Resumes in Norway: Neerali

Research and Development-Microbilogist Resume

Contact Candidate

Name:

Neerali

Location:

Norway

Experience:

Willing to Relocate:

Willing to Travel:

Most Recent Job Title:

Research and Development-Microbilogist

Personal Website:

Objective:

To contribut in the field of Research and Development whith development, standardization and validation of in-vitro assay for safety and efficacy of natural products which includes conducting primary research, critical analysis, and interpretation of test methods.

Resume Text:

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Education Details:

Examination College Year of passing Aggregate
Percentage
M.Sc.
Microbiology The Oxford College of Science, Bangalore University April 2001 71%
B.Sc
(Botany, Chem, Microbiology) Sri Bhagawan Mahaveer Jain College, Bangalore university April 1999 73%
PUC
(Phy, Chem, Bio, Comp.Sc.) St.Anne’s College, PU Board-Bangalore April 1996 59%

Employment History:

Company Name Designation Start date – End date
Sami Labs Limited, Bangalore Scientist
Since July 2003

Dayanand Sagar College, Bangalore Research Assistant Sept 2001 to June 2003

Project Details:

 Projects undertaken in Sami Labs, a subsidiary of Sabinsa Corporation, USA (http://www.sabinsa.com), listed among Inc 500 America’s fastest growing companies.

I. Screening of compounds for medicinal & cosmetic applications:

Antioxidant Activity:

 DPPH Scavenging Assay
 ORAC (Oxygen Radical Absorbing Capacity Assay)
 Lipid Peroxidation

II. Screening of compounds having Anti-Wrinkle Properties:

 Elastase inhibition assay
 Collagenase inhibition assay
 Hyaluronidase inhibition assay

III. Screening of compounds having Skin Lightening Properties:

 Mushroom Tyrosinase inhibition assay using tube and microplate method

IV. Screening of compounds for Anti-Obesity Properties:

 Lipase inhibition assay

V. Estimation of Gluten content in test materials.

VI. Genotoxicity Studies:

 Bacterial Reverse Mutation Test (Ames test/ BRMT) as per OECD Guidelines # 471:
Evaluation of the mutagenic potential of test articles using Salmonella typhimurium, viz., TA98, TA100, TA102, TA1535, TA1537 and Escherichia coli WP2 trp uvrA & pKM101 in presence and absence of Aroclor induced rat liver S9.
 Photomutagenicity Assay:
Evaluation of Photomutagenic potential of test articles using Salmonella typhimurium, TA102.
 Micronucleus Assay as per OECD Draft Guidelines # 487:
Human Lymphocytes.
Invitro Micronucleus Assay using CHO K1 Cell Line (OECD Draft Guidelines # 487).

VII. In vitro Proliferation/Cytotoxicity Assays

 In vitro Cytotoxicity Assay by Sulphorhodamine Blue (SRB) method.
 In vitro Cytotoxicity Assay using Trypan Blue Dye Exclusion method
 In vitro Cytotoxicity Assay using MTT assay

VIII. Anti-inflammatory Studies:
 Quantitative Determination of Human Prostaglandin E2 (PGE-2) levels in the plasma of Human volunteers (Psoriasis) treated with various test compounds using ELISA method.
 Quantitative Determination of Tumor Necrosis Factor (TNF alpha) levels in the plasma of Human volunteers (Psoriasis) treated with various test compounds using ELISA method.
 Quantitative Determination of Vascular Endothelial Growth Factor (VEGF) levels in the plasma of Human volunteers (Psoriasis) treated with various test compounds using ELISA method.

IX. Microbiological Projects:

1. Microbial Analytical Methods for testing Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella ebony.
2. Testing of compounds for Antimicrobial Activity using S. aureus, E. coli, S. mutans, albicans, Malassezia furfur.
3. Sterility testing for finished products.

4. Preservative Efficacy Testing using TTC and Alamar blue by Microplate Method (Fluorescence based assay)

X. Estimation of Color Index of various powders, extracts, solutions, creams and skin tanning using Minolta Chromameter (CM2600d)

Wet Lab Techniques:

a. Gene Cloning Techniques:
 Isolation of genomic and plasmid DNA from bacteria.
 Restriction Digestion of DNA.
 In vitro DNA ligation.
 Bacterial Transformation.
 Agarose Gel Electrophoresis.

b. Immunotechniques:

 Ouchterlony Double Diffusion (ODD).
 Radial Immunodiffusion (RID).
 Enzyme Linked Immunosorbent Assay (ELISA).
 Conter Current Immunoelectrophoresis (CCIEP).
 Pregnancy (hCG) Detection.
 Hepatitis (HBs Ag) Detection.
 Latex Agglutination Test.

 Projects Undertaken at Dayanand Sagar College:

1. Detection of Contamination in Aviation Fuels by Bioluminescence Technique for Centre of Military Airworthiness & Defence Research & Development Organization, Bangalore.
2. Developed Bio-Kits for detection of contamination in Aviation fuels.

 Projects Undertaken during M.Sc:

1. Lead Poisoning Prevention and treatment under the guidance of Dr. Venkatesh, Head of Dept., Biochemistry and Biophysics, St. Johns Medical College and Hospital, Bangalore.

2. Correlation of various tests for different samples of Raw Milk with keeping Quality of milk under the guidance of Dr. A. M. Natarajan, R&D Center, K. C. Das Pvt. Ltd., Bangalore.

Workshop and Training:

 Introduction and Updates for Good Laboratory Practice-A review of Quality Assurance and NABL Accredition Training Course for GLP, September, 2005 at Sami Labs

Other activities:

1. Preparation of Departmental SOP’s (Experimental, Instruments and General).
2. Preparation of Reports of actives for various assays.

Merit Awards:
1. Certificate from Institute of Biosciences and Molecular Biology, on practical experimental techniques.

2. Certificate from The George Foundation for participation and contribution at the International Conference on Lead Poisoning and Prevention.

3. Certificate of Merit for Excellence in Slide Identification from Microbiology Association of St. Joseph’s College, Bangalore.

General Expertise:

Evaluation of activities of compounds (statistical analysis) using various softwares like GraphPad Prism, MS Excel, Power point Presentation, Literature survey.

Expertise in using the following instruments:

• BMG Floustar Optima (Absorbance, Flourescence, Luminescence)
• Opsys Plate washer ( for microplate-ELISA kits)
• Ultracold Centrifuge
• Spectrophotometer
• Minolta Chromometer for color evaluation of powders creams, etc.
• Olympus Inverted microscope with Image analysis
• Leica Microscopes with ImagePro Software
• Sonicators, Colony Counters, B.O.D Incubator etc.

Personal Summary:

Date of Birth: 19.03.1979

Linguistic Skills: English, Gujarati, Kannada, Hindi

Marital Status: Married

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