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arti
India
MSc biotechnology and BSc biotechnology
To pursue a Research career in the field of Plant Molecular Biology and Recombinant DNA technology. To dedicate my knowledge for the welfare of society by implementing advance technology of science in food and medicine which are the essential commodities of humanity.
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in Biotechnology (2003-05)
• Consistent among top three rankers of Post Graduate College.
• Aggregate 77% in M. Sc. Degree from SRMC & RI Chennai.
Bachelor of Science in Industrial Microbiology (2000-03)
Aggregate 73% in B. Sc. Degree from Patna Women's College
Represented Patna women's college in intercollegiate meet for Science Students 2002 for paper presentation.
Skills
Microbiology Basic Microbiological Techniques.
Plant tissue Culture Micro propagation, Transformation using Agrobacterium tumefaciens
Bioprocess Technology Fermentation Technology.
Molecular Biology and Genetics Isolation of chromosomal and plasmid DNA, Quantification of DNA
Recombinant DNA Technology Transformation, Restriction digestion, Polymerase Chain Reaction, ISSR
Immunology Purification of Ig, Haemagglutination, Immunological techniques
Pharmaceutical Biotechnology Pharmacognosy, Thin layer Chromatography, Column Chromatography, Bioautography
Cell Biology Cell enumeration, Isolation of lymphocyte
Animal Tissue Culture Primary culture, Passaging, Cryopreservation, viability count
Biochemistry: Biochemical assay, Enzyme assay, Protein purification
Instrumentation Techniques UV-VIS Spectrophotometer Agarose gel electrophoresis SDS-PAGE electrophoresis Chromatography Thermocycler Rotary evaporator, Microscopy
Software Skills
BLAST, Linux,Unix
Vi editor
Shell programming and Perl Programming
Project Summary
“Studies on Swietenia mahogany (Meliaceae) - Tissue culture, Inter simple sequence repeat (ISSR) and phytochemical analysis”
In the Present study it is proposed to examine this tree species for tissue culture response- Callus Induction from root & leaves, Shoot elongation from nodes & seed germination. The genomic DNA was isolated by CTAB method & it was subjected to PCR to know the Inter Simple Sequence Repeats (ISSR) using five different primers (UBC 879, UBC 825, UBC 828, UBC 859, and UBC 860). Analysis of phyto-constiuents was done from leaf powder by using TLC. Antimicrobial activity of different constituents was checked against S. typhi & Cryptococus by Bioautography technique.
Awards received
Secured school First rank for secondary school leaving examination
Languages English, Hindi.
On Job Trainings
• Pathological & Microbiological training at Sen diagnosis, Patna , Bihar.
• 'Microbial characterization of industrial waste and nursery soil' at AIIMS, New Delhi.
Paper Presentation
• 'Microbial characterization of industrial waste and nursery soil' at Integrated science national meetfor college students 2002(ISNM'02), Chennai.
• 'Recent applications of monoclonal antibodies' at RENOUVEAU'04 at Hindustan college of Arts & Science, Chennai
References
Dr.T.S.Lokeshwari
Assistant professor and
Head of Department
Department of Biotechnology
Sri Ramachandra Medical College and Research Institute Chennai-41
Email-tsloki@yahoo.com Dr.Charles E. Jebraj
Assistant professor
Department of Biotechnology
Sri Ramachandra Medical College and Research Institute Chennai-41
Personal Date of Birth 16th July 1983.
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